Cell and Molecular Biology Research Methods, Protocols, Lab Techniques

Transfection.WS website - an educational Biology Transfection Research Resource

This portal is focused on Molecular and Cell Biology research methods, experimental protocols, and laboratory techniques: gene silencing and RNA Interference (RNAi), stem cells and cancer cell lines, in vitro protein expression, cell banking, plasmid DNA cloning, in vivo transfection kits, nanoparticles, transient and stable transfection, mRNA expression and qRT-PCR, siRNA/shRNA/microRNA transfection and gene expression, antibody production, liposome encapsulation, development of stable cell lines, electroporation and DNA fusion, in vivo siRNA transfection, contract laboratory service (CROs), cell-based assay development, xenograft animal model services, and many other topics.

The goal of this page is to collect standard protocols for a broad variety of microbiological and biochemical research techniques. Techniques covered by expert scientists include xenotransplantation for cancer, inflammatory, and other drug research, as well as gene silencing via RNAi effects, including siRNA and shRNA. Also discussed is inducible gene expression, protein production, cell signaling, transfection, the use of nanoparticles in biological research, and delivery of RNAi-species for therapeutic use.  In addition, this website boasts an exhaustive discussion of cell-based assays, as well as enzyme-linked immunosorbent assays (ELISA).

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Discussion Topics and Articles:

Apoptosis - just as all living beings are set to expire, cells in multicellular organisms that are also programmed to die. In the literature, this process of programmed cell death is referred to as apoptosis ... continued at http://www.transfection.ws/apoptosis   

Stem cells are undifferentiated cells that maintain capability to self-divide not only for renewal of its own type, but also to differentiate into certain subtypes (become specialized) under various conditions ... continued at http://www.transfection.ws/stem_cells_basics 

Molecular Biology is a field of Biology dealing with the study of molecules and molecular processes that form the underlying basis of life. The research focus of Molecular biology is proteins and nucleic acids - DNA and RNA. This science field includes and closely related to Biochemistry, Genetics, Gene Expression, Cell Biology, Microbiology, Pharmaceuticals, Bioengineering and Biotechnology. Also, in conjunction with computers and data analytics, has given rise to such fields as Computational Biology, Bioinformatics, Molecular Genetics ... continuied at http://www.transfection.ws/molecular_biology

Small interfering RNA (siRNA) also referred to as silencing RNA or short interfering RNA is used in the gene silencing technique to suppress gene expression. Typically consisting of 20-25 nucleotide double stranded RNA (dsRNA), siRNA is extensively used in RNA interference (RNAi) particularly in mammalian cells ... continued at  www.transfection.ws/sirna

Transfection is the process of introducing a purified segment of deoxyribonucleic acid (DNA) or ribonucleic acid (RNA) into a suitable eukaryotic cell. The term transfection is typically used when the process is performed in vitro uding cultured cells. The same procedure when done in plants, fungi, bacteria and algae is referred to as transformation (3). Transfection is one of the primary technologies used for studying genetics and devising gene therapy strategies ... continued at www.transfection.ws/transfection

Gene silencing, also termed RNA interference (RNAi), is the technique through which the expression of an otherwise "switched on" gene is suppressed. Though a gene may be silenced at transcriptional or post-transcriptional stages, RNAi is specifically post-transcriptional gene silencing mechanism achieved by introducing a double stranded RNA (dsRNA) ... continued at http://www.transfection.ws/gene_silencing

Cancer - appearance, disease description, and  DNA mechanism of action ... continued at http://www.transfection.ws/cancer_101

RNA Interference in animals - in vivo siRNA transfection to induce gene silencing ... continued at http://www.transfection.ws/rnai_in_animals

Transient and stable transfection - researchers use transfection technique to insert a segment of DNA or RNA into a host cell. After the insertion into cell, the external oligonucleotide may or may not become a part of the host cellular genome ... continued at http://www.transfection.ws/transient_and_stable_transfection

Links: Transfection Resource, In Vivo Transfection Reagents and Kits, Preclinical CRO Services, Stable Cell Line in 28 Days Service  

Cell and Molecular Biology Research Methods, Protocols, Lab TechniquesDeoxyribonucleic acid (DNA)RNA molecule and its function and structure microRNARNAi TherapeuticsRNAi In Animals: In Vivo siRNA and BiodistributionsiRNA function and screening servicesGene Expression Methods, Protocols, TechniquesAntigensGeneration of stably expressing cellsApoptosisCancer 101Cell Based AssaysCell BiologyCell Line Specific Transfection ReagentsCommercial RNAi ServicesCustom Life Science Research ServicesDNA TransfectionDNA Transfection ReagentElectroporationGeneration of Stable Cell LinesGene SilencingLipid Based Transfection ReagentsNanoparticle Based Transfection ReagentsPolymer Based Transfection ReagentsReverse TransfectionsiRNAsiRNA Transfection BasicsStem Cells BasicsTransfectionTransfection Products Offered by AltogenTransfection ServicesTransient and Stable TransfectionGene ExpressionMolecular Biology
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