Mycoplasma Contamination?

Mycoplasma Contamination?

Postby bioforum on Mon Jul 21, 2014 4:12 pm

We have a cell line in house that we suspect has been contaminated with mycoplasma. Is there a standard assay that gets used to determine this?
Posts: 10
Joined: Tue Jun 24, 2014 2:25 pm

Re: Mycoplasma Contamination?

Postby medicbio on Mon Jul 21, 2014 5:04 pm

There are several options:
-commercial kit: my lab has had good experiences with the e-myco plus mycoplasma kit, that uses PCR, made by iNtRoN Biotechnology. It’s a conventional PCR method with ready-to-use reagents and comes with a positive control, an extraction control, and an internal control for PCR inhibition. We’ve found it covers a broad spectrum of mycoplasma due to its primer selection for conserved 16S rRNA.
-nested PCR method: We have used a method from this paper with some success:
-a colleague has used a SYBR green rtPCR method, but our lab looked at it and thought it might be expensive and time-consuming, so we haven’t tried it.
Posts: 7
Joined: Tue Jun 24, 2014 2:23 pm

Return to General Topics

Who is online

Users browsing this forum: No registered users and 1 guest