Primer Size?

Primer Size?

Postby cell2bio on Fri Jul 18, 2014 4:01 pm

When designing primers, I use a lot of the online tools like primer3, fast pcr, and net primer - however, none of them show product size. How do you typically get the product size of your primers?
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Re: Primer Size?

Postby rtpcrman on Fri Jul 18, 2014 4:07 pm

When I do primer design in a tool like that, I grab the sequence, and paste it into MS Word. That way I can highlight it and annotate it. I use the find tool to find the primers in the sequence, highlight them, and then use the Word Count tool to determine character length, which in this case is your product size.
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