Research Methods, Experimental Protocols, and Laboratory Techniques
biologist wrote:Stable cell line creation is utilized for RNAi knockdown as well as gene expression and differentiation studies, inducible expression studies, as well as methods and techniques to utilize the transference of cell lines in vivo. Stable cell lines can be created through transfection of DNA plasmids or shRNA-expressing constructs. Selection or the use of certain virus particles will allow DNA integration into recipient cell genomes and help to reduce the formally long period of time that was necessary for the creation of a stable cell line.
Cell lines that have been stably expressed for the purposes of protein or RNAi sequencing is a precise an exact science that utilizes a variety of approaches. The approach is dependent upon the type of expression (constituted or inducible) that is desired. Results demand large-scale biochemical analysis of fusion proteins as well as monitoring of cell cycle localization.
Stably expressing cell lines are able to encode at least one antibiotic gene that is resistant to various drugs in order to enable drug selection processes. Through this process, transfected genetic materials may be expressed in specific target cells depending on investigative as well as methods utilized to determine short-term impact of protein expression or altered gene expression.
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