Recombinant stable cell line / RNAi cell lines

[siRNA]

Recombinant stable cell lines are important biological models used for research purposes, protein productionb and diagnostics. It usually takes 2-3 months to make a stable cell line, mostly due to low stable transfection efficiency and very low chance of correct integration of DNA fragment into host cell genome (genomic DNA). The process is very laborious and require cells become antibiotic-resistant in order to complete drug selection process.

Recently, scientists started to use stable cell lines for overexpression of shRNA (siRNA) in order to generate RNAi knockdown cell lines. This mechanism allows to induce stable gene silencing (RNAi) of specificly targeted gene (researchers often focus on oncogenes) in cultured cells.