Research Methods, Experimental Protocols, and Laboratory Techniques
siRNA wrote:Altogen Services provide liposome encapsulation services (http://www.altogenlabs.com). Liposomes are phospholipid structures that are capable to encapsulate various types of nucleic acids and small molecules (shRNA, siRNA, microRNA, plasmid DNA, mRNA, Protein) within the aqueous core of the liposome. Such liposomal complexes proivide protection and induce highly efficient transfection of cargo molecules (medicines, pharmaceuticals, small moledule druds, DNA, RNA, proteins) into cells in vitro and in vivo. Altogen Services (http://www.altogenlabs.com) offers encapsulation of any charged molecuIes into one of the standard liposomes (PC:Cholesterol, or DSPC:Lipid: PEG-Lipoid:Cholesterol) or into any custom liposome formulation. Microfluidization, sonication and liposome extrusion technologies are used to make liposomes of defined size (from 50 nm to 400 nm).
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