siRNA structure and activity

siRNA structure and activity

Postby biologist on Tue Oct 06, 2009 10:42 am

The structure of siRNA comprises of a 20-to 28-nucleotide long double-stranded RNA molecules. siRNAs may also be introduced into cells through manual or artificial means via various transaction methods. This type of process provides inactivation of a specific gene. Therefore, various genes can be targeted and have been studied for drug target validation studies as well as gene functions in genomic research. Inadequate introduction of siRNA can initiate immune responses that are undesirable, as well as those that may be focused toward specific processes in medicine and biology. To date, the effectiveness of siRNAs in different cell types is not fully understood, and while some cells respond well to them, others don't.
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Re: siRNA structure and activity

Postby postdoc on Wed Jul 06, 2011 7:40 pm

"Natural pathway" include siRNA entry to the cell, recognition by enzyme DICER, and processing into ~22 nucleotide duplexes.
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Re: siRNA structure and activity

Postby transfection on Wed Jul 06, 2011 7:47 pm

In mammalian cells introduction of long dsRNA leads to interferon/PKR response, however this can be avoided by instroduction of "short hairpin" or shRNA.
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Re: siRNA structure and activity

Postby postdoc on Sun Nov 27, 2011 9:37 am

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Re: siRNA structure and activity

Postby cell2bio on Wed Jul 02, 2014 5:01 pm

Gene inactivation occurs via formation of the RNA-induced silencing complex, or RISC. The siRNA is acted on by the Dicer enzyme, and then the RISC uses the original siRNA as a template to destroy any complementary mRNA, silencing the expression of that gene.
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