The structure of siRNA comprises of a 20-to 28-nucleotide long double-stranded RNA molecules. siRNAs may also be introduced into cells through manual or artificial means via various transaction methods. This type of process provides inactivation of a specific gene. Therefore, various genes can be targeted and have been studied for drug target validation studies as well as gene functions in genomic research. Inadequate introduction of siRNA can initiate immune responses that are undesirable, as well as those that may be focused toward specific processes in medicine and biology. To date, the effectiveness of siRNAs in different cell types is not fully understood, and while some cells respond well to them, others don't.